The role of nonspecific proteolysis in the development of alterative changes in lung tissue when using electronic cigarettes

In recent years, the number of electronic cigarettes (ES) users has been growing steadily around world. ES heat and vaporize components a mixture propylene glycol, vegetable glycerin, nicotine, flavors. Reports severe forms lung injury caused by use ES, some which have resulted in death, urge studying pathogenetic mechanisms such complications. One is activation nonspecific proteolysis accompanied destruction connective tissue blood-air barrier. Aim study . To dynamics proteinase-inhibitor system indicators its role morphological changes experimental modeling effects ES. Methods Experimental studies were performed 24 white male Wistar rats weighing 160 – 180 g. was simulated intermittent exposure to smoke vapors using POD connected two-channel compressor. Results. Analysis state proteinase inhibitory rat broncho-alveolar lavage blood serum results indicate trypsin-like proteinases depletion local antitryptic potential, leading biochemical with subsequent structural tissue. The latter are characterized congestion, leukocyte infiltration, stromal proliferation. addition, nicotine-containing mixtures shown affect composition, associated compensatory increase antitrypsin activity. Conclusion. lavage, damaging effect on tissue, disturbances hemodynamics, extravasation leukocytes, remodeling thickening